Transcriptomics

Dataset Information

3

Transcription profiling by array of Burkholderia cenocepacia J2315 grown under low or atmospheric oxygen concentration conditions


ABSTRACT: B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at OD 0.3 under full aeration and then transferred into a 50 ml centrifuge tube and placed upright into a CampyGen Compact (Oxoid, Basingstoke, UK) plastic pouch containing the gas generating paper sachet. The pouch was sealed immediately and then the cap of the centrifuge tube loosened to allow exchange of atmosphere between centrifuge tube and pouch.
The culture was further incubated at 37 degrees centigrade and 150 rpm for 2-3 hours until it reached OD 0.5. The centrifuge tube was sealed before opening the pouch and then snap-cooled before harvest to minimise exposure to atmospheric oxygen and to ensure expression profiles reflect the lower oxygen concentration.
The expression profile was compared to cells grown to OD 0.5 at atmospheric oxygen concentrations.

ORGANISM(S): Burkholderia cenocepacia  

SUBMITTER: Andrea M Sass  

PROVIDER: E-MEXP-2787 | ArrayExpress | 2012-01-01

REPOSITORIES: ArrayExpress

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Publications

The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia.

Sass Andrea M AM   Schmerk Crystal C   Agnoli Kirsty K   Norville Phillip J PJ   Eberl Leo L   Valvano Miguel A MA   Mahenthiralingam Eshwar E  

The ISME journal 20130314 8


Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying diverse environmental and pathogenic lifestyles. B. cenocepacia is known for its ability to cause lung infections in people with cystic fibrosis and it possesses a large 8 Mb multireplicon genome encoding a wide array of pathogenicity and fitness genes. Transcriptomic profiling across nine growth conditions was performed to identify the global gene expression changes made w  ...[more]

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