Transcriptomics

Dataset Information

8

Comparative genomic hybridization by array of mouse embryonic stem cells with giant piggyBac transposons stably inserted


ABSTRACT: Detection of copy-number gain of the ES cells with giant piggyBac transposons stably inserted in the genome using Agilent regional high density comparative genomic array with average probe spacing 130 bp. Extracts were made from equal amounts of human lymphoma cell line DNA mixed with mouse ES samples and control (wild type AB2.2) DNA in order to produce a baseline for normalisation for the array copy number detection.

ORGANISM(S): Musculus  

SUBMITTER: Tomas W Fitzgerald  

PROVIDER: E-MEXP-2788 | ArrayExpress | 2010-09-01

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-MEXP-2788.README.txt Txt
E-MEXP-2788.idf.txt Idf
E-MEXP-2788.idf.txt_original Idf
E-MEXP-2788.processed.1.zip Processed
E-MEXP-2788.raw.1.zip Raw
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Publications

Mobilization of giant piggyBac transposons in the mouse genome.

Li Meng Amy MA   Turner Daniel J DJ   Ning Zemin Z   Yusa Kosuke K   Liang Qi Q   Eckert Sabine S   Rad Lena L   Fitzgerald Tomas W TW   Craig Nancy L NL   Bradley Allan A  

Nucleic acids research 20110924 22


The development of technologies that allow the stable delivery of large genomic DNA fragments in mammalian systems is important for genetic studies as well as for applications in gene therapy. DNA transposons have emerged as flexible and efficient molecular vehicles to mediate stable cargo transfer. However, the ability to carry DNA fragments >10 kb is limited in most DNA transposons. Here, we show that the DNA transposon piggyBac can mobilize 100-kb DNA fragments in mouse embryonic stem (ES) ce  ...[more]

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