Name: Comparative genomic hybridization by array of mouse embryonic stem cells with giant piggyBac transposons stably inserted
Keywords: comparative genomic hybridization by array

Dataset Information

Comparative genomic hybridization by array of mouse embryonic stem cells with giant piggyBac transposons stably inserted

ABSTRACT: Detection of copy-number gain of the ES cells with giant piggyBac transposons stably inserted in the genome using Agilent regional high density comparative genomic array with average probe spacing 130 bp. Extracts were made from equal amounts of human lymphoma cell line DNA mixed with mouse ES samples and control (wild type AB2.2) DNA in order to produce a baseline for normalisation for the array copy number detection.

ORGANISM(S): Musculus

SUBMITTER: Tomas W Fitzgerald

PROVIDER: E-MEXP-2788 | ArrayExpress | 2010-09-01

REPOSITORIES: ArrayExpress

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Dataset's files

Source:

			Action	DRS
	E-MEXP-2788.README.txt	Txt
	E-MEXP-2788.idf.txt	Idf
	E-MEXP-2788.idf.txt_original	Idf
	E-MEXP-2788.processed.1.zip	Processed
	E-MEXP-2788.raw.1.zip	Raw

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Publications

Mobilization of giant piggyBac transposons in the mouse genome.

Li Meng Amy MA Turner Daniel J DJ Ning Zemin Z Yusa Kosuke K Liang Qi Q Eckert Sabine S Rad Lena L Fitzgerald Tomas W TW Craig Nancy L NL Bradley Allan A

Nucleic acids research 20110924 22

The development of technologies that allow the stable delivery of large genomic DNA fragments in mammalian systems is important for genetic studies as well as for applications in gene therapy. DNA transposons have emerged as flexible and efficient molecular vehicles to mediate stable cargo transfer. However, the ability to carry DNA fragments >10 kb is limited in most DNA transposons. Here, we show that the DNA transposon piggyBac can mobilize 100-kb DNA fragments in mouse embryonic stem (ES) ce ...[more]

PMID: 21948799

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Project description:Background Primitive brain tumors are the first cause of cancer-related death in children. Tumor cells with stem-like properties (TSCs), thought to account for tumorigenesis and therapeutic resistance, have been isolated from high-grade gliomas in adults. Whether TSCs are a common component of pediatric brain tumors and are of clinical relevance remains to be determined. Methodology/Principal findings Tumor cells with self-renewal properties were isolated with cell biology techniques from a majority of 55 pediatric brain tumors samples, regardless of their histopathologies and grades of malignancy (57% of embryonal tumors, 57% of low-grade gliomas and neuro-glial tumors, 70% of ependymomas, 91% of high-grade gliomas). The vast majority (10/12) of high-grade glioma-derived oncospheres sustained long-term self-renewal numbers akin to neural stem cells (>7 self-renewals), whereas cells with limited renewing abilities akin to neural progenitors dominated in all other tumor types. Regardless of tumor entities, the young age group was associated with self-renewal properties akin to neural stem cells (P=0.05, chi-square test). TSCs shared a complex molecular profile combining embryonic stem cell markers with elements controlling neural stem cell properties and epithelio-mesenchymal transitions. They were radio- and chemoresistant and formed aggressive tumors after intracerebral grafting. Survival analysis of the cohort showed an association between isolation of TSCs with long-term self-renewal abilities and a patients higher mortality rate (P = 0.022, log-rank test). Patients bearing cells with limited self-renewal properties constituted an intermediate group of survival but which did not reach statistical significance. Conclusions/Significance In brain tumors affecting adult patients, TSC have been isolated only from high-grade gliomas. In contrast, our data show that tumor cells with stem cell-like or progenitor-like properties can be isolated from a wide range of histological sub-types and grades of pediatric brain tumors. They suggest that cellular mechanisms fueling tumor development differ between adult and pediatric brain tumors.