ABSTRACT: Investigating the oxidative stress response: Candida glabrata strains were stressed with hydrogen peroxide and menadione (causing oxygen radicals) to induce the oxidative stress regulon, which is thought to be upregulated during the oxidative burst inside of phagocytic cells.
The human fungal pathogen Candida glabrata is related to Saccharomyces cerevisiae but has developed high resistance against reactive oxygen species. We find that induction of conserved genes encoding antioxidant functions is dependent on the transcription factors CgYap1 and CgSkn7 which cooperate for promoter recognition. Superoxide stress resistance of C. glabrata is provided by superoxide dismutase CgSod1, which is not dependent on CgYap1/Skn7. Only double mutants lacking both CgSod1 and CgYap ...[more]
Project description:DCP (2-4-dichlorophenol; 0,3mM) and POELE (polyoxyethylen-9-laurylether; 0,1mM)treatment on Saccharomyces cerevisiae W303-1A wild-type cells. Further investigation of the involvement of the transcripton factors Pdr1 and Pdr3 in DCP treated cells. Cells were growing in early exponential phase in rich medium.
Project description:Candida albicans wild type SC5314 and the eed1 delta mutant were used to infect reconstituted human oral epithelium (RHE) for 24h at 37°C and 5% CO2. Samples were taken 1h, 12h and 24h after infection. Total RNA was isolated, labeled with Cy5 and hybridised with a Cy3- labeled common reference.
Project description:C. albicans wild type strain SC5314, the eed1 deletion mutant and an eed1 delta mutant overexpressing UME6 (eed1 + pTET-UME6) were grown on plastic (37°C, RPMI1640 medium, plastic surface, 5% CO2) for 12h. Total RNA was isolated using a phenol-chloroform protocol and labeled with Cy5. Cy5- labeled sample RNA was hybridised with Cy3- labeled common reference (SC5314, 37°C, exponential culture).
Project description:Induction of apoptosis by the tumor suppressor p53 is known to protect from Myc-driven lymphomagenesis. The p53 family member p73 is also a pro-apoptotic protein, which is activated in response to oncogenes like Myc. We therefore investigated whether p73 provides a similar protection from Myc-driven lymphomas as p53. To generate B-cell lymphomas with defined genetic alterations in p53 or p73, we crossed the Eµ-Myc transgenic to mice heterozygous for germ-line deletions in p53 (p53+/) or p73 (p73+/-). Lymphomas which have spontaneously developed in Eµ-Myc transgenic animals with the genotypes p53+/+, p53+/-, p73+/+, p73+/- or p73-/- were isolated when the animals were moribund and further processed for gene expression profiling with 22.5K cDNA microarrays.
Project description:SH-EP cells were depleted for ZBTB4; Transfection of the cells were performed by oligofectamin RNAimax (Invitrogen) with siRNA smart pool (Dharmacon RNA Technologies); total RNA was isolated using RNAeasy Mini Kit (QIAGEN), preamplified, labeled with Cy3 and Cy5, respectively, and hybridized to cDNA-microarrays
Project description:Arabidopsis thaliana plants fend off insect attack by constitutive and inducible production of toxic metabolites such as glucosinolates (GS). A triple mutant lacking MYC2, MYC3, and MYC4, three basic helix-loop-helix transcription factors that are known to additively control jasmonate-related defense responses, is highly susceptible to insect herbivory. In this study, we performed a whole-genome microarray analysis on three-week-old plants and compared wild-type (Col-0) and myc2myc3myc4 plants in control conditions (no treatment). Myc234 was shown to have a highly reduced expression of GS biosynthesis genes.