Transcriptomics

Dataset Information

3

Transcription profiling by array of mouse M1 cells expressing Batf targeting siRNA or control siRNA and treated with leukemia inhibitory factor to identify putative downstream targets of Batf


ABSTRACT: In order to identify putative downstream targets of Batf in M1 cells, a microarray analysis was performed. Briefly, M1/Neg-RNAi (expressing control siRNA with no mammalian target) and M1/Batf-RNAi cells (expressing Batf targeting siRNA) were seeded in M1 growth medium at a density of 3 X 105/ml in 100mm culture dishes. Six plates were plated for each cell type. Within each group, three were labeled as + LIF and treated with leukemia inhibitory factor (LIF) (5 ng/ml) for 6 hours the following day. After 6 hours, the cells were harvested from all plates individually and RNA prepared. 5 µg of total RNA from the three individual samples representing each subgroup (M1/Neg-RNAi + LIF, - LIF; M1/Batf-RNAi + LIF, - LIF) were combined into one pool of RNA. EJT001 represents sample "M1/Neg-RNAi, no LIF)"; EJT002 represents sample "M1/Neg-RNAi, with LIF)"; EJT003 represents sample "M1/Batf-RNAi, no LIF)"; EJT004 represents sample "M1/Batf-RNAi, with LIF)". The four pools of RNA were shipped to Genome Explorations, Inc. for analysis using Affymetrix GeneChip technology. The differentially regulated genes were identified both within each group (+ LIF compared to - LIF) and between 2 goups (M1/Neg-RNAi and M1/Batf-RNAi).

INSTRUMENT(S): 418 [Affymetrix]

SUBMITTER: Juan Liao  

PROVIDER: E-MEXP-2995 | ArrayExpress | 2011-03-01

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-MEXP-2995.README.txt Txt
E-MEXP-2995.eSet.r Other
E-MEXP-2995.idf.txt Idf
E-MEXP-2995.processed.1.zip Processed
E-MEXP-2995.raw.1.zip Raw
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Publications

Batf promotes growth arrest and terminal differentiation of mouse myeloid leukemia cells.

Liao Juan J   Humphrey Sean E SE   Poston Stacie S   Taparowsky Elizabeth J EJ  

Molecular cancer research : MCR 20110204 3


Batf is a basic leucine zipper transcription factor belonging to the activator protein-1 superfamily. Batf expression is regulated following stimulation of both lymphoid and myeloid cells. When treated with leukemia inhibitory factor, mouse M1 myeloid leukemia cells commit to a macrophage differentiation program that is dependent on Stat3 and involves the induction of Batf gene transcription via the binding of Stat3 to the Batf promoter. RNA interference was employed to block Batf induction in t  ...[more]

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