Dataset Information


MicroRNA profiling by array of mouse lung from individuals sensitised with chicken egg OVA and then challenged with aerosolised OVA

ABSTRACT: specific pathogen-free female BALB/c mice aged 7-8 weeks (Animal Resources Centre, Perth, Western Australia) were systemically sensitised by intraperitoneal injection of 50 µg of alum-precipitated chicken egg OVA (Grade V, ?98% pure, Sigma Australia) 21 and 7 days before inhalational challenge, then exposed to aerosolised OVA in a whole body inhalation exposure chamber (Unifab Corporation, Kalamazoo, MI). Chronic low-level challenge involved exposure to ?3 mg/m3 aerosolised OVA for 30 minutes/day on 3 days/week for up to 6 weeks. Particle concentration within the chamber was continuously monitored using a DustTrak 8520 instrument (TSI, St Paul, MN). All experimental procedures complied with the requirements of the Animal Care and Ethics Committee of the University of New South Wales (reference numbers: 06/119B and 08/09B). Mice were sacrificed after 1,2,4 and 6 weeks of OVA exposure. Control groups included naïve mice and mice that were not sensitised but were challenged for 6 weeks with aerosolised OVA.

ORGANISM(S): Mus musculus  

SUBMITTER: Adam Collison  

PROVIDER: E-MEXP-3118 | ArrayExpress | 2011-06-10


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Altered expression of microRNA in the airway wall in chronic asthma: miR-126 as a potential therapeutic target.

Collison Adam A   Herbert Cristan C   Siegle Jessica S JS   Mattes Joerg J   Foster Paul S PS   Kumar Rakesh K RK  

BMC pulmonary medicine 20110523

BACKGROUND: The role of microRNAs (miRNAs) in regulating gene expression is currently an area of intense interest. Relatively little is known, however, about the role of miRNAs in inflammatory and immunologically-driven disorders. In a mouse model, we have previously shown that miRNAs are potentially important therapeutic targets in allergic asthma, because inhibition of miR-126, one of a small subset of miRNAs upregulated in the airway wall, effectively suppressed Th2-driven airway inflammation  ...[more]

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