Dataset Information


Transcription profiling by array of Aspergillus fumigatus wild type and srBA knock out strains grown under hypoxic conditions

ABSTRACT: 3 replicates of WT and srbA knockout strains grown on GMM plates, harvested spores and inoculated 5x10^5 spores/ml in 300 ml of LGMM. Cultures incubated for 10h at 28C and then shifted for 2h to 37C (both at 200rpm) so they are just germinating at the start of the treatment. Cultures were shifted to hypoxia (37C, 200rpm) and incubated for 1hr. Samples were collected by pouring them over a filter paper in a Buechner funnel and snap froze the cells immediately in liquid nitrogen, and stored the cells at -80C and lyophilized all tissue at the same time after all samples were collected. RNA extraction was then done simultaneously with all time points.

ORGANISM(S): Aspergillus fumigatus  

SUBMITTER: Bridget M Barker  

PROVIDER: E-MEXP-3172 | ArrayExpress | 2011-08-01


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SREBP coordinates iron and ergosterol homeostasis to mediate triazole drug and hypoxia responses in the human fungal pathogen Aspergillus fumigatus.

Blatzer Michael M   Barker Bridget M BM   Willger Sven D SD   Beckmann Nicola N   Blosser Sara J SJ   Cornish Elizabeth J EJ   Mazurie Aurelien A   Grahl Nora N   Haas Hubertus H   Cramer Robert A RA  

PLoS genetics 20111201 12

Sterol regulatory element binding proteins (SREBPs) are a class of basic helix-loop-helix transcription factors that regulate diverse cellular responses in eukaryotes. Adding to the recognized importance of SREBPs in human health, SREBPs in the human fungal pathogens Cryptococcus neoformans and Aspergillus fumigatus are required for fungal virulence and susceptibility to triazole antifungal drugs. To date, the exact mechanism(s) behind the role of SREBP in these observed phenotypes is not clear.  ...[more]

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