Dataset Information


Chromatin immunoprecipitation of Zea mays chloroplast extracts prepared from wild type and CAF1-deficient tissue

ABSTRACT: Immunoprecipitation of the chloroplast splicing factor CAF1 and analysis of bound RNAs with a complete Zea mays cpDNA microarray.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Zea mays  

SUBMITTER: Alice Barkan   Rosalind Williams-Carrier  Christian Schmitz-Linneweber 

PROVIDER: E-MEXP-318 | ArrayExpress | 2005-08-01


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RNA immunoprecipitation and microarray analysis show a chloroplast Pentatricopeptide repeat protein to be associated with the 5' region of mRNAs whose translation it activates.

Schmitz-Linneweber Christian C   Williams-Carrier Rosalind R   Barkan Alice A  

The Plant cell 20050902 10

Plant nuclear genomes encode hundreds of predicted organellar RNA binding proteins, few of which have been connected with their physiological RNA substrates and functions. In fact, among the largest family of putative RNA binding proteins in plants, the pentatricopeptide repeat (PPR) family, no physiologically relevant RNA ligands have been firmly established. We used the chloroplast-splicing factor CAF1 to demonstrate the fidelity of a microarray-based method for identifying RNAs associated wit  ...[more]

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