Transcriptomics

Dataset Information

46

ChIP-chip, RIP-chip and transcription profiling by array of human T-cells to identify the set of mRNAs that HIV-1 Tat interacts with in T-cells


ABSTRACT: We have used RNA immunoprecipitation to identify the set of mRNAs that HIV-1 Tat interacts with in T-cells. We have also performed measurements of relative RNA abundance to determine if Tat binding is associated with an increase in RNA abundance in Tat-expressing T-cells and during HIV infection of primary T-cells. We have also used RNA IP and ChIP-Chip to compare the RNAs with which Tat interacts with to the RNAs that RISC interacts with and the genes associated with pTEF-b.

ORGANISM(S): Homo sapiens  

SUBMITTER: Richard Jenner  

PROVIDER: E-MEXP-3199 | ArrayExpress | 2013-11-05

REPOSITORIES: ArrayExpress

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Publications

Human immunodeficiency virus Tat associates with a specific set of cellular RNAs.

Bouwman Russell D RD   Palser Anne A   Parry Chris M CM   Coulter Eve E   Rasaiyaah Jane J   Kellam Paul P   Jenner Richard G RG  

Retrovirology 20140703


Human Immunodeficiency Virus 1 (HIV-1) exhibits a wide range of interactions with the host cell but whether viral proteins interact with cellular RNA is not clear. A candidate interacting factor is the trans-activator of transcription (Tat) protein. Tat is required for expression of virus genes but activates transcription through an unusual mechanism; binding to an RNA stem-loop, the transactivation response element (TAR), with the host elongation factor P-TEFb. HIV-1 Tat has also been shown to  ...[more]

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