Project description:Relative H3K27me3 enrichment, assessed by ChIP-on-chip, in two TLX positive and two TLX negative T Acute Lymphoblastique Leukemia (T-ALL) samples. Acute lymphoblastic leukemias (ALLs) are characterized by multi-step oncogenic processes leading to cell differentiation arrest and proliferation. Specific abrogation of maturation blockage constitutes a promising therapeutic option in cancer, which requires precise understanding of the underlying molecular mechanisms. We show that the cortical thymic maturation arrest in T-lineage ALL that over-express TLX1 or TLX3 is due to binding of TLX1/TLX3 to ETS1, leading to repression of the T cell receptor (TCR) alpha enhanceosome activity and blocked TCR-Jalpha rearrangement. TLX1/TLX3 abrogation or enforced TCRalpha/beta expression leads to TCRalpha rearrangement and apoptosis. Importantly, the auto-extinction of clones carrying TCRalpha-driven TLX1 expression supports TLX 'addiction' in TLX-positive leukemias and provides further rationale for targeted therapy based on disruption of TLX1/TLX3.
Project description:The onset of an infection-specific transcriptional program precedes the clinical diagnosis in patients who developed Ventilator-associated pneumonia (VAP). Ventilator-associated tracheobronchitis (VAT) is another respiratory infection affecting<br><br>outcomes in intubated patients, but interactions between VAT and VAP remains unknown.
Project description:The oligo microarrays were used to compare gene expression profile among type 1 Diabetes Mellitus (T1DM), type 2 Diabetes Mellitus (T2DM), Gestational Diabetes Mellitus (GDM), and their respective control subjects.