Transcription profiling of Francisella tularensis subsp. holarctica LVS following oxidative stress
ABSTRACT: Francisella tularensis LVS was grown to mid log phase and culture split into two. One culture served as control and other added H2O2 to final concentration of 0.03%. After 20 min at 37 C, RNA was isolated from both cultures
Project description:Most sRNAs control the expression of target genes by interacting with their mRNA. The fvr1 (Francisella virulence RNA 1) gene is found in the IGR between FTL_0777 and FTL_0778 and its sequence does not overlap those of the flanking genes, indicating that its target(s) is located in trans. Base-pairing between a sRNA and target mRNA generally leads to changed translation and often the stability of the mRNA is affected as well. Therefore, to identify possible targets of Fvr1, we compared the transcriptomes of the LVS?fvr1(LVS, for Live Vaccine Strain) and LVS/pfvr1+ strains after growth in liquid broth.