Transcriptomics

Dataset Information

9

Genotyping by array of human papillomavirus (HPV) in patient cervical DNA samples to evaluate a HPV genotyping microarray method


ABSTRACT: Human papillomavirus infection is the cause of essentially all cases of cervical premalignant lesions and cervical cancer. Primary screening using HPV DNA testing with cytology triage has been shown to be more sensitive and, importantly, more specific than conventional testing based on cytology among women more than 35 years of age.



The aim of the experiment was to evaluate a HPV genotyping microarray method using patient sample DNA. HPV genotyping is based on multiplex PCR (PGMY-t primers) followed by ligation step where two probes are ligated together if a matching template is present in the mixture. The ligated probes are then detected on microarray.

ORGANISM(S): Human papillomavirus  

SUBMITTER: Jarmo Ritari  

PROVIDER: E-MEXP-3466 | ArrayExpress | 2011-12-10

REPOSITORIES: ArrayExpress

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Publications

Detection of human papillomaviruses by polymerase chain reaction and ligation reaction on universal microarray.

Ritari Jarmo J   Hultman Jenni J   Fingerroos Rita R   Tarkkanen Jussi J   Pullat Janne J   Paulin Lars L   Kivi Niina N   Auvinen Petri P   Auvinen Eeva E  

PloS one 20120323 3


Sensitive and specific detection of human papillomaviruses (HPV) in cervical samples is a useful tool for the early diagnosis of epithelial neoplasia and anogenital lesions. Recent studies support the feasibility of HPV DNA testing instead of cytology (Pap smear) as a primary test in population screening for cervical cancer. This is likely to be an option in the near future in many countries, and it would increase the efficiency of screening for cervical abnormalities. We present here a microarr  ...[more]

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