Transcriptomics

Dataset Information

14

Transcription profiling by array of human MKN74 gastric epithelial cells infected with Enterococcus faecalis for 24 hrs or 5day


ABSTRACT: MKN74 cells were infected for 24 h or 5 days. For the 24 h infection, 80% confluent MKN74 cells were washed three times in PBS and incubated in antibiotic free medium. Overnight-grown colonies of E. faecalis were collected and added to the MKN74 cell culture at a multiplicity of infection (MOI) of 50 bacteria per cell. Cultures were maintained at 37 C under a 5% CO2 humidified atmosphere. 5 day infections were carried out by treating 65% confluent MKN74 cells with E. faecalis at a MOI of 10. Every 24 h, cells were washed three times with PBS and fresh medium and bacteria were added. Control cells were processed similarly in the absence of bacteria.

REANALYSED by: E-MEXP-3496

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Jesper B Strickertsson  

PROVIDER: E-MEXP-3496 | ArrayExpress | 2012-04-02

REPOSITORIES: ArrayExpress

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Publications

Enterococcus faecalis infection causes inflammation, intracellular oxphos-independent ROS production, and DNA damage in human gastric cancer cells.

Strickertsson Jesper A B JA   Desler Claus C   Martin-Bertelsen Tomas T   Machado Ana Manuel Dantas AM   Wadstrøm Torkel T   Winther Ole O   Rasmussen Lene Juel LJ   Friis-Hansen Lennart L  

PloS one 20130430 4


BACKGROUND: Achlorhydria caused by e.g. atrophic gastritis allows for bacterial overgrowth, which induces chronic inflammation and damage to the mucosal cells of infected individuals driving gastric malignancies and cancer. Enterococcus faecalis (E. faecalis) can colonize achlohydric stomachs and we therefore wanted to study the impact of E. faecalis infection on inflammatory response, reactive oxygen species (ROS) formation, mitochondrial respiration, and mitochondrial genetic stability in gast  ...[more]

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