Dataset Information


Reproducibility M-Tram of Enterococcus faecium strain E1163

ABSTRACT: Two independent aliquots containing approximately 10^7 cells from the transposon mutant of E. faecium E1162 were grown at 37 C for 20 hours in 20 ml of BHI broth. Genomic DNA was isolated from the two replicate cultures and used for the generation of cDNA. The cDNA samples were labeled with Cy3 and Cy5 respectively and hybridized to a microarray that was designed using the E. faecium E1162 genome sequence.

ORGANISM(S): Enterococcus faecium  

SUBMITTER: Xinglin Zhang  

PROVIDER: E-MEXP-3502 | ArrayExpress | 2012-11-02


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Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium.

Zhang Xinglin X   Paganelli Fernanda L FL   Bierschenk Damien D   Kuipers Annemarie A   Bonten Marc J M MJ   Willems Rob J L RJ   van Schaik Willem W  

PLoS genetics 20120628 6

Enterococcus faecium has become a nosocomial pathogen of major importance, causing infections that are difficult to treat owing to its multi-drug resistance. In particular, resistance to the β-lactam antibiotic ampicillin has become ubiquitous among clinical isolates. Mutations in the low-affinity penicillin binding protein PBP5 have previously been shown to be important for ampicillin resistance in E. faecium, but the existence of additional resistance determinants has been suggested. Here, we  ...[more]

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