The presence of persister cells has been proposed as a factor in biofilm resilience. In the present study we investigated whether persister cells are present in Burkholderia cepacia complex (Bcc) biofilms, what the molecular basis of antimicrobial tolerance in Bcc persisters is, and how persisters can be eradicated from Bcc biofilms. After treatment of Bcc biofilms with high concentrations of various antibiotics often a small subpopulation survived. To investigate the molecular mechanism of tole ...[more]
Project description:Diploid human lung fibroblasts (WI-38 cells) were exposed to TGFbeta2 (inducing myofibroblast differentiation), GW501516 (a PPARbetadelta agonist) or both. Treated and control samples were hybridized against a reference sample made up of all samples pooled in equal parts and diluted by the number of samples.
Project description:RNA was purified from mouse embryonic fibroblast (MEF) and 3 embryonic stem (ES) cell lines after preplating. Total RNA was subjected to transcriptome analysis using Agilent Array- 028005:SurePrint G3 Mouse GE 8x60K Microarray. The common reference is a mixture of MEF, IB10c, HMSc1, HMSc2.
Project description:We observed extensive neurite formation in NG108-15 cells cultured in the presence of the flavonoid, isoquercitrin. To help determine the mechanism of neuritogenesis, microarray analysis was performed on samples treated with 40 uM isoquercitrin for 24 hrs.
Project description:The well-known colorectal adenoma-carcinoma sequence suggests that a normal epithelial cell, through accumulations of genetic lesion and epigenetic disregulation can transform into a benign adenoma then further develop into a cancer. Using microarray-based comparative genomic hybridization (CGH), we reveal genome-wide copy number variations in colorectal cancer and polyp and use them to determine the tissues clonal relationship.
Project description:This research was realized with the Mycelium Donor Plant (MDP) in vitro culture system (Voets et al., 2009), which allowed to synchronize the development of the AM fungus in the roots and to analyse the gene expression study particularly during the first stages (Gallou et al., 2010). In the present study, we investigated the global transcriptional change in the potato roots during the pré- (i.e. before contact of AM fungi with roots), early (i.e. first hyphae in the roots) and late (i.e. effective symbiosis with arbuscules in the roots) stages of Glomus sp. MUCL 41833 establishment.
Project description:G. max plants was grown in plastic pots filled with soil for 3 weeks under a 12 h light/12 h dark at 28°C. Cold treatment: The 3-week-old plants were transferred from 28°C to 4°C and were grown for 1 day. Dehydration treatment: The 3-week-old plants were grown for 4 days without watering.