Transcription profiling by array of C. albicans SC5314 culture to investigate response to mannich ketones
ABSTRACT: An exponentially growing C. albicans SC5314 culture in SD medium at 30 C was split into two flasks, one exposed to MIC90 concentration of compound 2 (6.25 ug./ml-1)mannich ketones, the other to the same volume of water. Samples were collected after 30 and 60 min for transcript profiling.
We investigated the antifungal activity of fused Mannich ketone (FMK) congeners and two of their aminoalcohol derivatives. In particular, FMKs with five-membered saturated rings were shown to have minimum inhibitory concentration (MIC90s) ranging from 0.8 to 6 µg/mL toward C. albicans and the closely related C. parapsilosis and C. krusei while having reduced efficacy toward C. glabrata and almost no efficacy against Aspergillus sp. Transcript profiling of C. albicans cells exposed for 30 or 60 m ...[more]
Project description:The study was conducted by comparing deleted and over-expression strains for SFL2 (CEC2001 and CEC1997, respectively) during a kinetic (0h, 2h and 4h) in YNB plus 2% casaminoacids (pPCK1- overexpression inducing conditions).
Project description:We performed a gene expression analysis of C. albicans SC5314 planktonic cells exposed to the antifungal peptide ApoEdpL-W. Exponentially-growing C. albicans SC5314 cells in SD at 30°C medium were exposed to 2.5 µM ApoEdpL-W and samples were collected after 10 and 30 min. for transcript profiling
Project description:The study was conducted by comparing deleted and over-expression strains for SFL1 (CEC1535 and CEC1509, respectively) during a kinetic (0h and 4h) in YNB plus 2% casaminoacids (overexpression pPCK1-inducing conditions).
Project description:Genome sequencing of species of the kinetoplastid parasite, Leishmania, that give rise to a range of disease phenotypes in the host has revealed highly conserved gene content and synteny across the genus. Only a small number of genes are differentially distributed between the three species studied to date, L. major, L. infantum and L. braziliensis. Here, we focus on RNA expression in the disease-promoting intracellular amastigotes and use customised oligonucleotide microarrays to confirm that all of these differentially-distributed genes are expressed in this critical stage of the parasite life cycle, with only a few regulated between species.
Project description:In this study, full human genome promoter microarrays and expression microarrays were used to explore the roles of histone modifications (H3K9Ac and H3K9Me2) upon the induction of MSC osteogenic differentiation.