Transcriptomics

Dataset Information

19

Transcription profiling by array of Arabidopsis seedlings grown in the dark and treated with epibrassinolide and glucose


ABSTRACT: imbibed Col-0 seeds were sown on ? X MS medium supplemented with 0.8% agar and 1% sucrose. The plates were first exposed to continuous light for 12 h to stimulate germination and then wrapped with two layers of aluminum foil and placed in the growth chamber for 5 d. Once the plant material was uniformly germinated, the experimental conditions were applied. 5-d-old, dark-grown seedlings were washed seven times with sterile water followed by a wash with ? X MS liquid medium without sucrose to remove residual exogenous sugar and the plant material was kept in ? X MS liquid without sucrose in the dark for all subsequent steps. Cultures were shaken at 140 rpm at 22 C for 24 h and then treated with ? X MS without glucose or ½ X MS supplemented with BR (100 nM), glucose (3%), or glucose (3%) + BR (100 nM) for 3 h. Seedlings were harvested after 3h and preceded for RNA isolation and microarray analysis.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Ashverya Laxmi  

PROVIDER: E-MEXP-3545 | ArrayExpress | 2012-08-10

REPOSITORIES: ArrayExpress

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Publications

Hypocotyl directional growth in Arabidopsis: a complex trait.

Gupta Aditi A   Singh Manjul M   Jones Alan M AM   Laxmi Ashverya A  

Plant physiology 20120611 4


The growth direction of the Arabidopsis (Arabidopsis thaliana) etiolated-seedling hypocotyl is a complex trait that is controlled by extrinsic signals such as gravity and touch as well as intrinsic signals such as hormones (brassinosteroid [BR], auxin, cytokinin, ethylene) and nutrient status (glucose [Glc], sucrose). We used a genetic approach to identify the signaling elements and their relationship underlying hypocotyl growth direction. BR randomizes etiolated-seedling growth by inhibiting ne  ...[more]

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