Transcription profiling by array of Bacillus amyloliquefaciens strain FZB42 after interaction exudates (IE) treatment at log phase(OD600=1.0) and early stationary phase(OD600=3.0)
ABSTRACT: Transcription profiling by array of Bacillus amyloliquefaciens strain FZB42 after interaction exudates (IE) treatment, at OD600=1.0 and at OD600=1.0 respectively. IE was the root exudates prepared from maize plants growing with FZB42. The reference was treated with the root exudates (RE), prepared from maize plants grown in an axenic system.
BACKGROUND: Plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates. RESULTS: Bacillus amyloliquefaciens FZB42 is a wel ...[more]
Project description:The transcriptomic response of Bacillus amyloliquefaciens FZB42 to maize root exudates at OD600=3.0. This is a comprehensive analysis using the data of six microarray experiments (Exp1-2-3 and ExpABC respectively, 18 hybridization in total).
Project description:Transcriptional profiling by array of Bacillus amyloliquefaciens FZB42 at log phase(OD600=1.0) and early stationary phase(OD600=3.0) after maize root exudate treatment of three concentrations(0.25mg/ml,0.50mg/ml,1.00mg/ml).
Project description:The effect of maize seed (Zea mays L. var. Surprise) exudates on Bacillus amyloliquefaciens FZB42 cultures grown to OD600 = 1.0 or 3.0 was tested against a negative seed exudate control sample.
Project description:Transcription profiles of Bacillus amyloliquefacs FZB42 (cultured to an optical densit of 1.0 or 3.0) in response to root exudates from nutrient-sufficient maize plants and plants starved of nitrogen, phosphorus, potassium or iron.
Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard <br>(University Aarhus, Denmark) and then self-propagated at the University of Seville.<br> Ljgln2-2 mutant was isolated from photorespiratory mutant screening as described <br>previously (Orea et al., 2002; Mï¿½rquez et al., 2005; Betti et al., 2006). <br>The mutant progeny of two consecutive back-crosses with WT was used<br> for the present work. WT and mutant seeds were scarified and surface-sterilized,<br> germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture<br> of vermiculite and sand as solid support. <br>Five seedlings were planted in each pot and grown <br>during 35 days in a growth chamber under 16/8 hours<br> day/night, 20/18ï¿½C, with a photosynthetic photon flux <br>density of 250 ï¿½mol/m2ï¿½s and a constant humidity of 70%.<br> CO2 was automatically injected to a final concentration of 0.7% (v/v),<br> to allow for normal growth of Ljgln2-2 mutant in a photorespiration <br>suppressed atmosphere. Plants were watered with Hornum nutrient solution <br>(Handberg & Stougaard, 1992).<br> Drought was applied withholding irrigation for the reported period <br>of time and sample plants or leaves were harvested for further analysis.
Project description:Gene expression in wildtype Rhizobium leguminosarum biovar viciae strain 3841 was comapred to a bacA mutant. All cultures were grown in the laboratory on AMS with glucose and ammonia as carbon and nitrogen sources.