Project description:Cells were starved for leucine in the presence or absence of a dominant negative HSF1 mutant (dnHSF1, HSF379). Changes in transcriptome relative to cells cultured with leucine were determined. The experiment shows the effect of dnHSF on the amino acid starvation response.
Project description:RNA was purified from mouse embryonic fibroblast (MEF) and 3 embryonic stem (ES) cell lines after preplating. Total RNA was subjected to transcriptome analysis using Agilent Array- 028005:SurePrint G3 Mouse GE 8x60K Microarray. The common reference is a mixture of MEF, IB10c, HMSc1, HMSc2.
Project description:We observed extensive neurite formation in NG108-15 cells cultured in the presence of the flavonoid, isoquercitrin. To help determine the mechanism of neuritogenesis, microarray analysis was performed on samples treated with 40 uM isoquercitrin for 24 hrs.
Project description:Transcriptional profiling of e8.5 mouse embryos comparing wt with Srd5a3Gt(betaGeo)703Lex/Gt(betaGeo)703Lex. Goal was to determine the developmental pathway disrupted in the mutant.<br>
Project description:The well-known colorectal adenoma-carcinoma sequence suggests that a normal epithelial cell, through accumulations of genetic lesion and epigenetic disregulation can transform into a benign adenoma then further develop into a cancer. Using microarray-based comparative genomic hybridization (CGH), we reveal genome-wide copy number variations in colorectal cancer and polyp and use them to determine the tissues clonal relationship.
Project description:G. max plants was grown in plastic pots filled with soil for 3 weeks under a 12 h light/12 h dark at 28°C. Cold treatment: The 3-week-old plants were transferred from 28°C to 4°C and were grown for 1 day. Dehydration treatment: The 3-week-old plants were grown for 4 days without watering.
Project description:Diploid human lung fibroblasts (WI-38 cells) were exposed to TGFbeta2 (inducing myofibroblast differentiation), GW501516 (a PPARbetadelta agonist) or both. Treated and control samples were hybridized against a reference sample made up of all samples pooled in equal parts and diluted by the number of samples.