Transcriptomics

Dataset Information

5

Transcription profiling of Arabidopsis thioredoxin reductase knock-out mutant


ABSTRACT: Transcriptomic study of a thioredoxin reductase knock-out mutant.

Biological question :
Thioredoxins are small redox proteins implicated in crucial pathways of cell life. They are reduced by a conserved flavoprotein named NADPH-dependent thioredoxin reductase (NTR). In order to give an insight into the functions of the NTR/thioredoxin pathway in Arabidopsis, we have used a reverse genetic approch. Both NTR genes (NTRA and NTRB) have been inactivated in the double ntra ntrb mutant. T-DNA lines have been provided by the SALK library. Surprisingly, this mutant shows a limited phenotype suggested that some additional redox pathways are involved in compensating the inactivation of the NTR/thioredoxin pathway. In order to isolate compensatory genes that would be transcriptionally induced in the ntra ntrb mutant, we have performed this CATMA project.

Seeds of the wild-type Col-0 ecotype and of the ntra ntrb homozygote double mutant were sowed in soil. Three replicates of lines were made (Col-0 1, 2 and 3 ntra ntrb 1, 2 and 3) : plants were put at different places of the greenhouse and grown at different times. Germination was synchronized by 4 days at 4C and plants were grown during 17 days, until they reached a 6 rosette leaves stage. Entire plants (30 plants per sample) were harvested, frozen in liquid nitrogen and stored at -80C until extraction.

INSTRUMENT(S): Axon GenePix 4000A scanning hardware

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Ludivine Taconnat  

PROVIDER: E-MEXP-365 | ArrayExpress | 2005-09-15

REPOSITORIES: ArrayExpress

Similar Datasets

2005-08-26 | E-MEXP-259 | ArrayExpress
2007-07-01 | E-MEXP-801 | ArrayExpress
2006-12-20 | E-MEXP-517 | ArrayExpress
2006-06-01 | E-MEXP-373 | ArrayExpress
2006-12-19 | E-MEXP-516 | ArrayExpress
2005-12-01 | E-MEXP-416 | ArrayExpress
2007-06-19 | E-MEXP-795 | ArrayExpress
2006-10-30 | E-MEXP-780 | ArrayExpress
2007-06-19 | E-GEOD-7998 | ArrayExpress
2007-01-26 | E-MEXP-573 | ArrayExpress