Transcriptomics

Dataset Information

4

The dependence on NFAT levels discriminates conventional T cells from Foxp3 expressing regulatory cells


ABSTRACT: In this study, we used microarrays to investigate the gene expression program in conventional T cells, nTreg and conventional T cells treated with TGFbeta (iTreg) from wild-type mice and and mice having NFAT1/NFAT2 double-deficient (DKO) T cells. CD4+CD25- and CD4+CD25+ T cells were isolated by MACS and stimulated for 24 h with anti-CD3 and anti-CD28 antibodies in the absence or presence of TGFbeta. RNA was extracted and microarray analyses were performed. The data represents two independent biological replicates.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Gerd Mueller  

PROVIDER: E-MEXP-3687 | ArrayExpress | 2012-10-15

REPOSITORIES: ArrayExpress

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Publications

Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells.

Vaeth Martin M   Schliesser Ulrike U   Müller Gerd G   Reissig Sonja S   Satoh Kazuki K   Tuettenberg Andrea A   Jonuleit Helmut H   Waisman Ari A   Müller Martin R MR   Serfling Edgar E   Sawitzki Birgit S BS   Berberich-Siebelt Friederike F  

Proceedings of the National Academy of Sciences of the United States of America 20120918 40


Several lines of evidence suggest nuclear factor of activated T-cells (NFAT) to control regulatory T cells: thymus-derived naturally occurring regulatory T cells (nTreg) depend on calcium signals, the Foxp3 gene harbors several NFAT binding sites, and the Foxp3 (Fork head box P3) protein interacts with NFAT. Therefore, we investigated the impact of NFAT on Foxp3 expression. Indeed, the generation of peripherally induced Treg (iTreg) by TGF-β was highly dependent on NFAT expression because the ab  ...[more]

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