Transcriptomics

Dataset Information

6

Transcription profiling by array of transgenic tobacco plants expressing begomoviral AC2 RNA silencing suppressor to compare transcriptional changes between transgenic and wild type plants


ABSTRACT: Transgenic tobacco plants expressing begomoviral AC2 RNA silencing suppressor were used to compare transcriptional changes in the transcriptome between transgenic and wild type tobacco plants. Transcriptional analysis using Agilent 4x44k tobacco array was performed of six week-old leaves and 3-5 month-old flowers taken from the same plants as leaf samples.

ORGANISM(S): Nicotiana tabacum  

SUBMITTER: Arto J Soitamo  

PROVIDER: E-MEXP-3724 | ArrayExpress | 2012-11-08

REPOSITORIES: ArrayExpress

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Publications

Expression of geminiviral AC2 RNA silencing suppressor changes sugar and jasmonate responsive gene expression in transgenic tobacco plants.

Soitamo Arto J AJ   Jada Balaji B   Lehto Kirsi K  

BMC plant biology 20121107


BACKGROUND: RNA-silencing is a conserved gene regulation and surveillance machinery, which in plants, is also used as major defence mechanism against viruses. Various virus-specific dsRNA structures are recognized by the silencing machinery leading to degradation of the viral RNAs or, as in case of begomoviruses, to methylation of their DNA genomes. Viruses produce specific RNA silencing suppressor (RSS) proteins to prevent these host defence mechanisms, and as these interfere with the silencing  ...[more]

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