Transcriptomics

Dataset Information

4

Transcription profiling by array of virus-infected Brachypodium distachyon to gain insight into the affected host molecular pathways upon infection with Panicum Mosaic Virus (PMV) together with its satellite virus, Satellite Panicum Mosaic Virus (SPMV)


ABSTRACT: We used Brachypodium distachyon (BD21) as a model grass to gain insight into the affected host molecular pathways upon infection of Panicum Mosaic Virus (PMV) together with its satellite virus, Satellite Panicum Mosaic Virus (SPMV). Brachypodium plants at 2-3 leaf stage were either mock inoculated or inoculated with PMV and PMV+SPMV. Total RNA was isolated from shoot tissues of control and treated plants and was subjected to microarray analysis.

ORGANISM(S): Brachypodium distachyon  

DISEASE(S): Pmv Infected,Control,Pmv Spmv Infected

SUBMITTER: Kranthi Mandadi  

PROVIDER: E-MEXP-3729 | ArrayExpress | 2012-11-09

REPOSITORIES: ArrayExpress

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Publications

Characterization of a viral synergism in the monocot Brachypodium distachyon reveals distinctly altered host molecular processes associated with disease.

Mandadi Kranthi K KK   Scholthof Karen-Beth G KB  

Plant physiology 20120906 3


Panicum mosaic virus (PMV) and its satellite virus (SPMV) together infect several small grain crops, biofuel, and forage and turf grasses. Here, we establish the emerging monocot model Brachypodium (Brachypodium distachyon) as an alternate host to study PMV- and SPMV-host interactions and viral synergism. Infection of Brachypodium with PMV+SPMV induced chlorosis and necrosis of leaves, reduced seed set, caused stunting, and lowered biomass, more than PMV alone. Toward gaining a molecular underst  ...[more]

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