Transcriptomics

Dataset Information

44

Transcription profiling by array of D. melanogaster to investigatepulse labeling and fractionation of RNA after inhibition of miR-277 to detect transcriptional and post-transcriptional changes


ABSTRACT: Drosophila melanogaster Schneider cells (S2) were transfected with a 2'-O-methyl inhibitor antisense to miR-277 or an unrelated control sequence. Three days after inhibition of the miRNA, the cellular RNA was pulse-labeled with 4-thio-Uridine for 1 hour, then tthe cells were lysed and RNA was extracted with Trizol. Following extraction, the RNA was further fractionated by in vitro biotinylation of incorporated 4-thio-uridine, yielding altogether three RNA fractions for each sample: total, newly transcribed (=labeled) and > 1h old (=unlabeled).

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Klaus Foerstemann  

PROVIDER: E-MEXP-3784 | ArrayExpress | 2013-11-25

REPOSITORIES: ArrayExpress

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Publications


Development, growth and adult survival are coordinated with available metabolic resources, ascertaining that the organism responds appropriately to environmental conditions. MicroRNAs are short (21-23 nt) regulatory RNAs that confer specificity on the RNA-induced silencing complex (RISC) to inhibit a given set of mRNA targets. We profiled changes in miRNA expression during adult life in Drosophila melanogaster and determined that miR-277 is downregulated during adult life. Molecular analysis rev  ...[more]

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