Transcriptomics

Dataset Information

6

Transcription profiling by array of human BIN-67 small cell ovarian carcinoma to investigate gene expression


ABSTRACT: The biology of small cell ovarian carcinoma of the hypercalcemic type (SCCOHT), which is a rare and aggressive form of ovarian cancer, is poorly understood. Tumourigenicity, in vitro growth characteristics, genetic and genomic anomalies, and sensitivity to standard and novel chemotherapeutic treatments were investigated in the unique SCCOHT cell line, BIN-67, to provide further insight in the biology of this rare type of ovarian cancer. Microarray expression analysis was performed using the GeneChip? Human Genome U133 Plus 2.0 Array (Affymetrix?, Santa Clara, CA) with total RNA from BIN-67 cells. Hybridization and scanning were performed at the McGill University and Genome Quebec Innovation Centre (Montreal, QC). Gene expression levels were determined from the scanned images using Affymetrix? Microarray Suite (MAS) version 5.0 software expression algorithm (Affymetrix?, Santa Clara, CA). Gene expression profiles were compared with Affymetrix U133 Plus 2.0 (MAS5.0) generated expression profiles (similarly normalized) from 10 human normal ovarian surface epithelial (OSE) cell brushings available in the ArrayExpress database (www.ebi.ac.uk/arrayexpress/), accession number E-GEOD-18520.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Suzanna Arcand  

PROVIDER: E-MEXP-3872 | ArrayExpress | 2013-09-24

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-MEXP-3872.idf.txt Idf
E-MEXP-3872.processed.1.zip Processed
E-MEXP-3872.raw.1.zip Raw
E-MEXP-3872.sdrf.txt Txt
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