Transcriptomics

Dataset Information

4

Transcription profiling by array of tobacco infected with tobacco mosaic virus or expressing the whole tobacco mosaic virus genome.


ABSTRACT: Agilent 4x44k tobacco micro array of wild type tobacco (WT) and whole tobacco mosaic virus (TMV) containing transgenic tobacco plants. The transgenic plants before resistance break (BRB-6 weeks), after resistance break (ARB-8 weeks) and wild type tobacco plants infected with TMV (TMVi-9weeks) leaves were analyzed. Three biological replicates were performed for each sample.

ORGANISM(S): Nicotiana tabacum  

SUBMITTER: Arto J Soitamo  

PROVIDER: E-MEXP-3934 | ArrayExpress | 2014-11-03

REPOSITORIES: ArrayExpress

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Publications

Multiple different defense mechanisms are activated in the young transgenic tobacco plants which express the full length genome of the Tobacco mosaic virus, and are resistant against this virus.

Jada Balaji B   Soitamo Arto J AJ   Siddiqui Shahid Aslam SA   Murukesan Gayatri G   Aro Eva-Mari EM   Salakoski Tapio T   Lehto Kirsi K  

PloS one 20140922 9


Previously described transgenic tobacco lines express the full length infectious Tobacco mosaic virus (TMV) genome under the 35S promoter (Siddiqui et al., 2007. Mol Plant Microbe Interact, 20: 1489-1494). Through their young stages these plants exhibit strong resistance against both the endogenously expressed and exogenously inoculated TMV, but at the age of about 7-8 weeks they break into TMV infection, with typical severe virus symptoms. Infections with some other viruses (Potato viruses Y, A  ...[more]

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