Transcriptomics

Dataset Information

8

Transcription profiling of Arabidopsis in the presence of sucrose to assess tolerance to the herbicide atrazine


ABSTRACT: Growth in the presence of sucrose was shown to confer to Arabidopsis thaliana seedlings, under conditions of in vitro culture, a very high level of tolerance to the herbicide atrazine and to other photosynthesis inhibitors (Sulmon et al., 2004). The CATMA investigation will be useful to reveal the gene networks implicated in the mechanisms of xenobiotics tolerance. Seeds of Arabidopsis thaliana (ecotype Columbia) were surface-sterilized inbayrochlore/ethanol (1:1, v/v), rinsed in absolute ethanol and dried overnight. Germination and growth were carried out under axenic conditions in square Petri dishes. After seeds were sowed, Petri dishes were placed at 4 C for 48 h in order to break dormancy and homogenize germination, and then transferred to a control growth chamber at 22°C under a 16-h light period regime at 4500 lux for 4 weeks. Growth medium consisted of 0.8% (w/v) agar in 1x Murashige and Skoog (MS) basal salt mix (Sigma, St. Louis, MO, USA) adjusted to pH 5.7. After 4 weeks of cultivation on vertical plates plantlets were transferred on fresh medium complemented or not with atrazine 10 uM and with sucrose 80mM or mannitol 80 mM which were directly added during preparation of agar-MS media prior to sterilisation. Atrazine was sterilized by microfiltration through 0.2 um cellulose acetate filters (Polylabo, Strasbourg, France) and then axenically added to melted agar-MS medium prior to pouring into Petri dishes. Then plantlets were harvested after 24 hours of transfer and extracted for RNA.

INSTRUMENT(S): Axon GenePix 4000A scanning hardware

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Ludivine Taconnat  

PROVIDER: E-MEXP-411 | ArrayExpress | 2006-08-23

REPOSITORIES: ArrayExpress

altmetric image

Publications

Genome-wide interacting effects of sucrose and herbicide-mediated stress in Arabidopsis thaliana: novel insights into atrazine toxicity and sucrose-induced tolerance.

Ramel Fanny F   Sulmon Cécile C   Cabello-Hurtado Francisco F   Taconnat Ludivine L   Martin-Magniette Marie-Laure ML   Renou Jean-Pierre JP   El Amrani Abdelhak A   Couée Ivan I   Gouesbet Gwenola G  

BMC genomics 20071205


BACKGROUND: Soluble sugars, which play a central role in plant structure and metabolism, are also involved in the responses to a number of stresses, and act as metabolite signalling molecules that activate specific or hormone-crosstalk transduction pathways. The different roles of exogenous sucrose in the tolerance of Arabidopsis thaliana plantlets to the herbicide atrazine and oxidative stress were studied by a transcriptomic approach using CATMA arrays. RESULTS: Parallel situations of xenobiot  ...[more]

Publication: 1/2

Similar Datasets

2007-03-10 | E-MEXP-574 | ArrayExpress
2005-08-26 | E-MEXP-259 | ArrayExpress
2007-06-19 | E-MEXP-795 | ArrayExpress
2006-12-19 | E-MEXP-516 | ArrayExpress
2007-07-01 | E-MEXP-801 | ArrayExpress
2005-09-15 | E-MEXP-365 | ArrayExpress
2007-01-26 | E-MEXP-573 | ArrayExpress
2006-06-01 | E-MEXP-373 | ArrayExpress
2005-12-01 | E-MEXP-416 | ArrayExpress
2006-12-20 | E-MEXP-517 | ArrayExpress