Transcriptomics

Dataset Information

4

Transcription profiling of Arabidopsis microRNA mutants, microRNA mutants, agrobacterium induced tumours, tasiRNA mutants and biotic stress treatements


ABSTRACT: Experiment 1
Gene profiling upon biotic stress treatment
Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment?
Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse.

Experiment 2
Gene profiling in microRNA mutants
Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants?
Experiment description : Ler, dcl1-9, hen1-1 and hasty were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after.

Experiment 3
Gene profiling in siRNA mutants
Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants?
Experiment description : Col-0, dcl2-1, dcl3-1 and rdr2-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after.

Experiment 4
Gene profiling in Agrobacterium-induced tumors and auxin-induced calli.
Biological question : What are the genes (including miRNA precursors) that are differentially regulated in an Agrobacterium-induced tumors compared to auxin-induced calli?
Experiment description : Col-0 plants were infected with wt Agrobacterium tumefaciens (strain A208) by stabbing the emerging stems. 18 days later, growing tumors were harvested as were the non-infected part of the stabbed-stem.In parallel, Col-0 seeds were germinated on Callus Induction Media (CIM) and 18 days later, calli were harvested.

Experiment 5
Gene profiling in tasiRNA mutants
Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of tasiRNA mutants?
Experiment description : Col-0, dcl4-1, rdr6-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after.

Experiment 6
Gene profiling upon biotic stress treatment
Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment?
Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse.


INSTRUMENT(S): Axon GenePix 4000A scanning hardware

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: ludivine taconnat  

PROVIDER: E-MEXP-517 | ArrayExpress | 2006-12-20

REPOSITORIES: ArrayExpress

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