Transcriptomics

Dataset Information

477

RNAi knock down of RNA binding IMP proteins in human epithelial (HeLa) cells


ABSTRACT: To identify factors and pathways regulated by IMP proteins and obtain leads to the mechanism behind the phenotypic changes, we compared the gene expression profiles of IMP siRNA treated cells with mock treated cells. Triplicate gene expression profiles were generated from both the IMP(1,3)A and IMP(1,3)B siRNA sets and were compared to the mock transfected cells. cRNA was hybridized to Affymetrix human U133A arrays.

REANALYSED by: E-MEXP-548

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Rehannah H Borup   Jonas Vikesaa  Thomas O Hansen 

PROVIDER: E-MEXP-548 | ArrayExpress | 2006-09-30

REPOSITORIES: ArrayExpress

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Publications

RNA-binding IMPs promote cell adhesion and invadopodia formation.

Vikesaa Jonas J   Hansen Thomas V O TV   Jønson Lars L   Borup Rehannah R   Wewer Ulla M UM   Christiansen Jan J   Nielsen Finn C FC  

The EMBO journal 20060316 7


Oncofetal RNA-binding IMPs have been implicated in mRNA localization, nuclear export, turnover and translational control. To depict the cellular actions of IMPs, we performed a loss-of-function analysis, which showed that IMPs are necessary for proper cell adhesion, cytoplasmic spreading and invadopodia formation. Loss of IMPs was associated with a coordinate downregulation of mRNAs encoding extracellular matrix and adhesion proteins. The transcripts were present in IMP RNP granules, implying th  ...[more]

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