Transcriptomics

Dataset Information

0

Transcription profiling of Arabidopsis Col-0 plants with wild type, arr1-1 knock-out, ARR1 over-expressor and ARR1-SRDX fusion protein over-expressor genotypes treated with 6-Benzyladenine for different lengths of time


ABSTRACT: Arabidopsis thaliana Col-0 plants and three other genotypes (ARR1 overexpressor, arr1-1 knockout, overexpressor of ARR1-SRDX fusion protein) were grown in liquid media (1/2 MS, 1 g/L sucrose, 0.5 g/L MES, pH 5.7) in a Percival AR-66L growth chamber at 24 oC, 16:8 h day:night cycle, and 100 µE light intensity until growth stage 1.0. Plants were then treated with 5 µM 6-Benzyladenine for 0, 15, and 120 min, harvested and frozen in liquid nitrogen for RNA extraction and subsequent processing for microarray hybridization.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Wolfram Brenner   

PROVIDER: E-MEXP-689 | ArrayExpress | 2008-08-31

REPOSITORIES: ArrayExpress

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Publications

Summarizing and exploring data of a decade of cytokinin-related transcriptomics.

Brenner Wolfram G WG   Schmülling Thomas T  

Frontiers in plant science 20150217


The genome-wide transcriptional response of the model organism Arabidopsis thaliana to cytokinin has been investigated by different research groups as soon as large-scale transcriptomic techniques became affordable. Over the last 10 years many transcriptomic datasets related to cytokinin have been generated using different technological platforms, some of which are published only in databases, culminating in an RNA sequencing experiment. Two approaches have been made to establish a core set of c  ...[more]

Publication: 1/2

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