Transcription profiling of Arabidopsis Col-0 plants with wild type, arr1-1 knock-out, ARR1 over-expressor and ARR1-SRDX fusion protein over-expressor genotypes treated with 6-Benzyladenine for different lengths of time
ABSTRACT: Arabidopsis thaliana Col-0 plants and three other genotypes (ARR1 overexpressor, arr1-1 knockout, overexpressor of ARR1-SRDX fusion protein) were grown in liquid media (1/2 MS, 1 g/L sucrose, 0.5 g/L MES, pH 5.7) in a Percival AR-66L growth chamber at 24 oC, 16:8 h day:night cycle, and 100 µE light intensity until growth stage 1.0. Plants were then treated with 5 µM 6-Benzyladenine for 0, 15, and 120 min, harvested and frozen in liquid nitrogen for RNA extraction and subsequent processing for microarray hybridization.
The genome-wide transcriptional response of the model organism Arabidopsis thaliana to cytokinin has been investigated by different research groups as soon as large-scale transcriptomic techniques became affordable. Over the last 10 years many transcriptomic datasets related to cytokinin have been generated using different technological platforms, some of which are published only in databases, culminating in an RNA sequencing experiment. Two approaches have been made to establish a core set of c ...[more]
Project description:6 days old Arabidopsis thaliana of Col-0 wild-type and 35S:ARR1-SRDX transgenic seedlings grown in liquid culture (1/2 MS, 1 g/L sucrose, 0.5 g/L MES, pH 5.7) were induced with either 5 µM 6-Benzyladenine (a cytokinin analog) for 15 or 120 min, or mock-treated 120 min as a control. These samples were subjected to microarray analysis.
Project description:Arabidopsis thaliana Col-0 wildtype plants were grown on soil (Jiffy7 peat pellets 42 mm) to the respective developmental stage (1.04 or 1.08 Boyes scale). The plants were sprayed with 100 µM Naphtylacetic acid and incubated for 15 min or 2 hours under normal growth conditions. Leaves 1 and 2 were harvested and immediately frozen in liquid nitrogen.
Project description:Arabidopsis Col-0 seedlings grown in liquid culture to growth stage 1.0 were treated with 5 µM Benzyladenine and incubated for the indicated time points. Roots and shoots of the seedlings were then harvested separately by taking each individual seedling with a soft forceps at the hypocotyl, dipping it into liquid nitrogen and breaking off the root at the inner wall of a collection tube that was swimming in liquid nitrogen. The shoot part was then striped from the forceps at the edge of another collection tubes.
Project description:Arabidopsis thaliana Col-0 wildtype plants were grown on soil (Jiffy7 peat pellets 42 mm) to the respective developmental stage (1.04 or 1.08 Boyes scale). The plants were sprayed with 50 µM Abscisic acid and incubated for 15 min or 2 hours under normal growth conditions. Leaves 1 and 2 were harvested and immediately frozen in liquid nitrogen.