Transcriptomics

Dataset Information

3

Transcription profiling of Strongyloides ratti larval stage 1 (L1) vs infective larval stage 3 (iL3)


ABSTRACT: Isofemale line ED321 Heterogonic was used. Fresh faeces were collected from S. ratti-infected rats at days 5, 6, 7 and 8 p.i. and L1s prepared with a Baermann funnel held for 6 h at 19oC. The larvae were concentrated by centrifugation and then cleaned by flotation on 60% v/v sucrose. Infective L3s were harvested from 14 day-old faecal cultures that had been maintained at 19oC. The iL3s were cleaned by sucrose flotation as for the L1s, above. In excess of 150,000 larvae of either stage were routinely isolated from 6 infected hosts. The experimental design used, was to have at least three biological replicates for each sample (i.e. three independent preparations of the relevant worm samples and their RNA) and to have at least three technical replicates (i.e. independent, separate cDNA synthesis, amplification and hybridization etc.) for each biological replicate. For each hybridisation a dye-swap was used i.e. each sample to be used in a hybridisation was labelled, separately, with each of the two dyes.

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Strongyloides ratti  

SUBMITTER: Mark E Viney   Clare P Wilkes  Jane Coghill  Gary L Barker  Louise Hughes  Fiona J Thompson 

PROVIDER: E-MEXP-697 | ArrayExpress | 2005-01-01

REPOSITORIES: ArrayExpress

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Publications

A microarray analysis of gene expression in the free-living stages of the parasitic nematode Strongyloides ratti.

Thompson Fiona J FJ   Barker Gary L A GL   Hughes Louise L   Wilkes Clare P CP   Coghill Jane J   Viney Mark E ME  

BMC genomics 20060619


The nematode Strongyloides ratti has two adult phases in its lifecycle: one obligate, female and parasitic and one facultative, dioecious and free-living. The molecular control of the development of this free-living generation remains to be elucidated.We have constructed an S. ratti cDNA microarray and used it to interrogate changes in gene expression during the free-living phase of the S. ratti life-cycle. We have found very extensive differences in gene expression between first-stage larvae (L  ...[more]

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