American journal of physiology. Lung cellular and molecular physiology 20070427 2
Transforming growth factor-alpha (TGF-alpha) and its receptor, the epithelial growth factor receptor (EGFR), have been associated with lung remodeling in premature infants with bronchopulmonary dysplasia (BPD). The goal of this study was to target TGF-alpha overexpression to the saccular phase of lung morphogenesis and determine early alterations in gene expression. Conditional lung-specific TGF-alpha bitransgenic mice and single-transgene control mice were generated. TGF-alpha overexpression wa ...[more]
Project description:The placenta plays a crucial role in the normal growth and development in mammals by serving as an interface for nutrients, respiratory gases and physiological signals between the mother and fetus. Here we use a novel embryo transfer system in mice to identify the major physiological pathways in the placenta that are principally influenced by altered maternal environment. Embryos of identical genotype were transferred at the one cell stage into surrogate mothers either of the same strain or from a different strain. We analyzed the relative effects of maternal and fetal genotype on fetal weight, placental weight and the placental transcriptome at E18.5. The results show that maternal genotype overrides fetal genotype as the principal regulator of fetal weight (p<0.0001). Microarray analysis of the transcriptome in placentas revealed that a small fraction (0.25%) of placental genes are specifically regulated by maternal genotype (p<0.05, FDR<0.10). Pathway analysis of these genes using the programs Gene Ontology and MetaCore from GeneGO inc. revealed highest statistical significance in signaling pathways that regulate cell growth and lipid metabolism. These results provide a mechanistic understanding of important molecular pathways involved in maternal regulation of fetal growth and development.
Project description:Evaluate the protective effect of prior tBHQ treatment from arsenic damage at two dosage levels of arsenic (2 and 20 uM), and compare to the effect of tBHQ or arsenic treatment given alone.
Project description:MEKK1 wildtype and knockout keratinocytes were compared both with and without activin (10 ng/ml) treatment for 12 hours, and control vs activin treated cells were compared both for wildtype and knockout. This was performed in a square design.
Project description:Role of the Ah-receptor in cell cycle regulation. Mouse fibroblasts expressing the Ah-receptor of C57Bl/6 mice under the regulation of the Tet*Off system were compared to Ahr-/- fibroblasts synchronized at G0/G1, S-phase, or in exponential growth.