Transcriptomics

Dataset Information

5

Transcription profiling of Strongyloides ratti larval stage 2 (L2) direct vs indirect development


ABSTRACT: S. ratti Isofemale line ED321 Heterogonic predominantly undergoes indirect development; isofemale line ED5 Homogonic predominantly undergoes direct development. Therefore, L2 stages of ED321 Heterogonic and of ED5 Homogonic are destined for indirect (i.e. L2 indirect) and direct (i.e. L2 direct) development, respectively; these sources of material were used in this comparison. To do this, for both isofemale lines, rats were infected with ED321 Heterogonic or ED5 Homogonic and faeces collected on days 5, 6, 7 and 8 p.i. and cultured for 24 h at 19oC, after which larvae were prepared with a Baermann funnel held for 6 h at 19oC, larvae were concentrated and cleaned by sucrose flotation, as above. In excess of 75,000 worms were routinely isolated from three infected hosts. The experimental design used, was to have at least three biological replicates for each sample (i.e. three independent preparations of the relevant worm samples and their RNA) and to have at least three technical replicates (i.e. independent, separate cDNA synthesis, amplification and hybridization etc.) for each biological replicate. For each hybridisation a dye-swap was used i.e. each sample to be used in a hybridisation was labelled, separately, with each of the two dyes.

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Strongyloides ratti  

SUBMITTER: Mark E Viney   Clare P Wilkes  Jane Coghill  Gary L Barker  Louise Hughes  Fiona J Thompson 

PROVIDER: E-MEXP-709 | ArrayExpress | 2005-01-01

REPOSITORIES: ArrayExpress

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Publications

A microarray analysis of gene expression in the free-living stages of the parasitic nematode Strongyloides ratti.

Thompson Fiona J FJ   Barker Gary L A GL   Hughes Louise L   Wilkes Clare P CP   Coghill Jane J   Viney Mark E ME  

BMC genomics 20060619


The nematode Strongyloides ratti has two adult phases in its lifecycle: one obligate, female and parasitic and one facultative, dioecious and free-living. The molecular control of the development of this free-living generation remains to be elucidated.We have constructed an S. ratti cDNA microarray and used it to interrogate changes in gene expression during the free-living phase of the S. ratti life-cycle. We have found very extensive differences in gene expression between first-stage larvae (L  ...[more]

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