Transcriptomics

Dataset Information

62

Transcription profiling by array of Saccharomyces cerevisiae after limitation of ammonium, L-alanine, L-glutamine or glucose


ABSTRACT: Response of Saccharomyces cerevisiae to Ammonium, L-alanine, or L-glutamine Limitation. The protrotophic laboratory strain CEN.PK113-7D (MAT a) was grown in laboratory fermentors with a working volume of 1 litre at dilution rate (D) of 0.20 per hour (in triplicate for each nitrogen limited condition). At steady state, samples from each of the 12 continuous cultures were taken and cooled below 2 degree C within ten seconds by mixing 40% sample and 60% crushed ice.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Jens Nielsen   Birgitte Regenberg  Renata Usaite  Thomas Grotkjaer  Kiran R Patil 

PROVIDER: E-MEXP-724 | ArrayExpress | 2006-06-13

REPOSITORIES: ArrayExpress

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Publications

Global transcriptional and physiological responses of Saccharomyces cerevisiae to ammonium, L-alanine, or L-glutamine limitation.

Usaite Renata R   Patil Kiran R KR   Grotkjaer Thomas T   Nielsen Jens J   Regenberg Birgitte B  

Applied and environmental microbiology 20060901 9


The yeast Saccharomyces cerevisiae encounters a range of nitrogen sources at various concentrations in its environment. The impact of these two parameters on transcription and metabolism was studied by growing S. cerevisiae in chemostat cultures with l-glutamine, l-alanine, or l-ammonium in limitation and by growing cells in an excess of ammonium. Cells grown in l-alanine-limited cultures had higher biomass yield per nitrogen mole (19%) than those from ammonium-limited cultures. Whole-genome tra  ...[more]

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