Transcriptomics

Dataset Information

50

Transcription profiling of Arabidopsis seedlings under conditions of salt stress for 6, 24 and 48 hours


ABSTRACT: Hydroponic cultured Arabidopis thaliana(ecotype Col-0) seeldings(18 days after germination)were treated with 1/2 X MS supplemented with 150 mM NaCl for 6, 24 and 48h, with roots harvested and pooled for RNA extraction. Control roots (1/2 X MS only)were also harvested at the same time for each time-point. 3 independent biological replicates were prepared for each time-point. Altogether 18 hybridizations including dye flips were performed with Qiagen Operon V1.0.2 26k microarray. Microarray data were analyzed using TIGR TM4 suite.

INSTRUMENT(S): arrayWoRx Biochip Reader [Applied Precision]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Michael Deyholos   Yuanqing Jiang 

PROVIDER: E-MEXP-754 | ArrayExpress | 2006-08-23

REPOSITORIES: ArrayExpress

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Publications

Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes.

Jiang Yuanqing Y   Deyholos Michael K MK  

BMC plant biology 20061012


BACKGROUND: Roots are an attractive system for genomic and post-genomic studies of NaCl responses, due to their primary importance to agriculture, and because of their relative structural and biochemical simplicity. Excellent genomic resources have been established for the study of Arabidopsis roots, however, a comprehensive microarray analysis of the root transcriptome following NaCl exposure is required to further understand plant responses to abiotic stress and facilitate future, systems-base  ...[more]

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