Transcriptomics

Dataset Information

70

Transcription profiling of Arabidopsis wild type, AtVdac1 knock-outs, p26 knock-outs and AtVdac1-p26 double kock-outs in response to growth in medium with salicylic acid


ABSTRACT: Functions of AtVDAC1 and its partner p26 in Arabidopsis thaliana. We previously showed that knock-out mutants impaired in AtVdac1 and p26 (AtVdac1 protein partner) are hypersensitive to salicylic acid: the root growth is more inhibited in response to 30 uM salicylic acid (SA) compared to the wild type. We want to know now which genes are up- or down-regulated in the mutants in control and SA conditions.
We want to compare the RNAm contents in the wild type, vdac1 and p26 mutants and, vdac1-p26 double mutant. One-week old plantlets were transferred for 2 hours on a fresh medium containing either 0 or 30uM salicylic acid.
Arabidopsis lines (WT, vdac1.1, p26.1, vdac1.1-p26.1) were grown in vertical position on ABIS medium (Lanquar et al., 2006). One week-old seedlings were transferred on a fresh ABIS medium containing either 0 or 30 uM salicylic acid. After 2 hours, the all seedlings were harvested. Three replicates were done; total RNAs from these 3 experiments were mixed together for the transcriptomic analysis.

INSTRUMENT(S): Axon GenePix 4000A scanning hardware

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: ludivine taconnat  

PROVIDER: E-MEXP-795 | ArrayExpress | 2007-06-19

REPOSITORIES: ArrayExpress

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Publications

Phosphatidylinositol 4-kinase activation is an early response to salicylic acid in Arabidopsis suspension cells.

Krinke Ondrej O   Ruelland Eric E   Valentová Olga O   Vergnolle Chantal C   Renou Jean-Pierre JP   Taconnat Ludivine L   Flemr Matyás M   Burketová Lenka L   Zachowski Alain A  

Plant physiology 20070511 3


Salicylic acid (SA) has a central role in defense against pathogen attack. In addition, its role in such diverse processes as germination, flowering, senescence, and thermotolerance acquisition has been documented. However, little is known about the early signaling events triggered by SA. Using Arabidopsis (Arabidopsis thaliana) suspension cells as a model, it was possible to show by in vivo metabolic phospholipid labeling with (33)P(i) that SA addition induced a rapid and early (in few minutes)  ...[more]

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