Transcriptomics

Dataset Information

67

Chromatin immunoprecipitation to identify H-NS and RNA polymerase (beta-subunit) binding sites on the Salmonella typhimurium chromosome


ABSTRACT: Chromatin immunoprecipitation experiment to identify H-NS and RNA polymerase (beta-subunit) binding sites on the Salmonella Typhimurium chromosome

INSTRUMENT(S): other

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium  

SUBMITTER: Gary Rowley   Jay C Hinton  Martin D Goldberg  Sacha Lucchini  Marcus Harrison  Douglas Hurd 

PROVIDER: E-MEXP-806 | ArrayExpress | 2006-08-17

REPOSITORIES: ArrayExpress

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Publications

H-NS mediates the silencing of laterally acquired genes in bacteria.

Lucchini Sacha S   Rowley Gary G   Goldberg Martin D MD   Hurd Douglas D   Harrison Marcus M   Hinton Jay C D JC  

PLoS pathogens 20060801 8


Histone-like nucleoid structuring protein (H-NS) is a modular protein that is associated with the bacterial nucleoid. We used chromatin immunoprecipitation to determine the binding sites of H-NS and RNA polymerase on the Salmonella enterica serovar Typhimurium chromosome. We found that H-NS does not bind to actively transcribed genes and does not co-localize with RNA polymerase. This shows that H-NS principally silences gene expression by restricting the access of RNA polymerase to the DNA. H-NS  ...[more]

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