Transcriptomics

Dataset Information

182

Transcription profiling of Arabidopsis or Thlaspi caerulescens roots exposed to deficient, sufficient or excess zinc


ABSTRACT: A common reference model was used to design the microarray experiment, in which cDNA from T. caerulescens roots exposed to 100 uM (sufficient) zinc was used as the common reference. Every slide was always hybridized with the common reference sample and with a sample from one of the treatments (Arabidopsis or T. caerulescens exposed to deficient, sufficient or excess zinc). The common reference was labeled with the fluorescent dye Cyanine 3 (Cy3) and the treatment samples were labeled with Cyanine 5 (Cy5). As a quality control step we performed a dye-swap hybridization for one sample (from T. caerulescens roots exposed to sufficient zinc).

INSTRUMENT(S): G2565BA DNA microarray scanner [Agilent]

SUBMITTER: Judith E van de Mortel   Laia Almar Villanueva  Perry D Moerland  Jeroen Kwekkeboom  Henk Schat  Sean Coughlan  Mark Aarts  Emiel Ver Loren van Themaat  Maarten Koornneef 

PROVIDER: E-MEXP-877 | ArrayExpress | 2006-10-10

REPOSITORIES: ArrayExpress

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Publications

Large expression differences in genes for iron and zinc homeostasis, stress response, and lignin biosynthesis distinguish roots of Arabidopsis thaliana and the related metal hyperaccumulator Thlaspi caerulescens.

van de Mortel Judith E JE   Almar Villanueva Laia L   Schat Henk H   Kwekkeboom Jeroen J   Coughlan Sean S   Moerland Perry D PD   Ver Loren van Themaat Emiel E   Koornneef Maarten M   Aarts Mark G M MG  

Plant physiology 20060922 3


The micronutrient zinc has an essential role in physiological and metabolic processes in plants as a cofactor or structural element in 300 catalytic and noncatalytic proteins, but it is very toxic when available in elevated amounts. Plants tightly regulate their internal zinc concentrations in a process called zinc homeostasis. The exceptional zinc hyperaccumulator species Thlaspi caerulescens can accumulate up to 3% of zinc, but also high amounts of nickel and cadmium, without any sign of toxic  ...[more]

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