Dataset Information

Transcription profiling of synchronized Sulfolobus acidocaldarius populations as they progress through the cell cycle

ABSTRACT: Exponentially growing Sulfolobus acidocaldarius were treated with NaAc to generate replication runout and arrest in G2 phase. The cells were then resuspended in fresh acetate-free media which generates a synchronous population. Samples for investigation of gene expression change were taken during the synchronised populations progress through the cell cycle.

INSTRUMENT(S): GenePix Personal 4100A [Axon Instruments]

ORGANISM(S): Sulfolobus acidocaldarius

SUBMITTER: Magnus Lundgren

PROVIDER: E-MEXP-936 | ArrayExpress | 2007-12-08

REPOSITORIES: ArrayExpress

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Publications

A unique cell division machinery in the Archaea.

Lindås Ann-Christin AC Karlsson Erik A EA Lindgren Maria T MT Ettema Thijs J G TJ Bernander Rolf R

Proceedings of the National Academy of Sciences of the United States of America 20081105 48

In contrast to the cell division machineries of bacteria, euryarchaea, and eukaryotes, no division components have been identified in the second main archaeal phylum, Crenarchaeota. Here, we demonstrate that a three-gene operon, cdv, in the crenarchaeon Sulfolobus acidocaldarius, forms part of a unique cell division machinery. The operon is induced at the onset of genome segregation and division, and the Cdv proteins then polymerize between segregating nucleoids and persist throughout cell divis ...[more]

PMID: 18987308

Publication: 1/2

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