Transcriptomics

Dataset Information

0

Chromatin immunoprecipitation of human breast cancer cell line MCF-7 treated with 17beta-estradiol


ABSTRACT: ChIP-DSL experiments using ER alpha antibody on v1 20k promoter array

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Homo sapiens  

DISEASE(S): Adenocarcinoma

SUBMITTER: Kasey R Hutt  

PROVIDER: E-MEXP-984 | ArrayExpress | 2007-03-31

REPOSITORIES: ArrayExpress

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Publications

Sensitive ChIP-DSL technology reveals an extensive estrogen receptor alpha-binding program on human gene promoters.

Kwon Young-Soo YS   Garcia-Bassets Ivan I   Hutt Kasey R KR   Cheng Christine S CS   Jin Mingjie M   Liu Dongyan D   Benner Chris C   Wang Dong D   Ye Zhen Z   Bibikova Marina M   Fan Jian-Bing JB   Duan Lingxun L   Glass Christopher K CK   Rosenfeld Michael G MG   Fu Xiang-Dong XD  

Proceedings of the National Academy of Sciences of the United States of America 20070314 12


ChIP coupled with microarray provides a powerful tool to determine in vivo binding profiling of transcription factors to deduce regulatory circuitries in mammalian cells. Aiming at improving the specificity and sensitivity of such analysis, we developed a new technology called ChIP-DSL using the DNA selection and ligation (DSL) strategy, permitting robust analysis with much reduced materials compared with standard procedures. We profiled general and sequence-specific DNA binding transcription fa  ...[more]

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