Transcriptomics

Dataset Information

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RNAseq analysis of global gene expression patterns in Corynebacterium glutamicum Adaptive Laboratory Evolution (ALE) strains GluA T5 and GluA T7 with accelerated growth and glutarate production.


ABSTRACT: The Adaptive Laboratory Evolution (ALE) experiment allowed to select Corynebacterium glutamicum strain GluA T5, which grew faster and produced more glutarate than the parent strain GluA T0, and strain GluA T7 that grew as fast as GluA T5, but produced more 5AVA than GluA T5. To explore differences in the gene expression in the evolved strains, C. glutamicum GluA T0, GluA T5 and GluA T7 biological triplicates were grown in CGXII minimal medium with 4% (w/v) glucose supplemented with 1 mM IPTG. Exponentially growing cells were harvested by centrifugation (14000 × g, 1 min) and kept at -80°C. RNA isolation, purification and quality control was performed as described [82] and the high quality RNA (RNA integrity number > 9.0) was kept at -80°C until further use. Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the ribosomal RNA molecules from the isolated total RNA. Preparation of cDNA libraries were performed according to the manufacturer’s instructions of TruSeq stranded mRNA Kit (Illumina, San Diego, USA). Subsequently, each cDNA library was sequenced on a HiSeq1500 (2 x 70nt PE rapid v2) and NextSeq 500 (2 x 75nt PE mid output v2.5) Sequencer system (Illumina, San Diego, USA).

INSTRUMENT(S): Illumina HiSeq 1500, NextSeq 500

ORGANISM(S): Corynebacterium glutamicum ATCC 13032  

SUBMITTER: Volker Wendisch   Carina Prell   Tobias Busche  

PROVIDER: E-MTAB-10025 | ArrayExpress | 2021-01-31

SECONDARY ACCESSION(S): ERP126458

REPOSITORIES: ArrayExpress, ENA

Dataset's files

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Action DRS
E-MTAB-10025.idf.txt Idf
E-MTAB-10025.sdrf.txt Txt
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Publications

Efficient Production of the Dicarboxylic Acid Glutarate by <i>Corynebacterium glutamicum</i> via a Novel Synthetic Pathway.

Pérez-García Fernando F   Jorge João M P JMP   Dreyszas Annika A   Risse Joe Max JM   Wendisch Volker F VF  

Frontiers in microbiology 20181030


The dicarboxylic acid glutarate is an important building-block gaining interest in the chemical and pharmaceutical industry. Here, a synthetic pathway for fermentative production of glutarate by the actinobacterium <i>Corynebacterium glutamicum</i> has been developed. The pathway does not require molecular oxygen and operates via lysine decarboyxylase followed by two transamination and two NAD-dependent oxidation reactions. Using a genome-streamlined L-lysine producing strain as basis, metabolic  ...[more]

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