Dataset Information


ChIP-seq of Pol II Rpb3 in male and female MSL2 RNAi Drosophila melanogaster salivary glands and S2 cell line

ABSTRACT: We performed Pol II ChIP-seq experiments with 3rd instar larva salivary glands material from male, female and MSL2 RNAi D.melanogaster, as well as from S2 cell lines material. The antibody used recognizes the Rpb3 subunit of RNA Pol II irrespective of the phosphorylation state of the Rpb1 carboxy-terminal domain, therefore capturing Pol II during all stages of transcription. Corrected processed data has been made available following Vaquerizas et al (2013). [Response to Comments on Drosophila Dosage Compensation Involves Enhanced Pol II Recruitment to Male X-Linked Promoters, Juan M. Vaquerizas, Florence M. G. Cavalli, Thomas Conrad, Asifa Akhtar, Nicholas M. Luscombe, Science 340 (6130): 273 (2013)]

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Florence M.G Cavalli  

PROVIDER: E-MTAB-1112 | ArrayExpress | 2012-07-27



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Drosophila dosage compensation involves enhanced Pol II recruitment to male X-linked promoters.

Conrad Thomas T   Cavalli Florence M G FM   Vaquerizas Juan M JM   Luscombe Nicholas M NM   Akhtar Asifa A  

Science (New York, N.Y.) 20120719 6095

Through hyperacetylation of histone H4 lysine 16 (H4K16), the male-specific lethal (MSL) complex in Drosophila approximately doubles transcription from the single male X chromosome in order to match X-linked expression in females and expression from diploid autosomes. By obtaining accurate measurements of RNA polymerase II (Pol II) occupancies and short promoter-proximal RNA production, we detected a consistent, genome-scale increase in Pol II activity at the promoters of male X-linked genes. Mo  ...[more]

Publication: 1/2

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