Transcriptomics

Dataset Information

240

Transcription profiling by array of Entamoeba histolytica trophozoites during growht under different iron concentrations


ABSTRACT: Entamoeba histolytica is the parasite causing amoebiasis, an infectious disease targeting the intestine and liver of humans. The molecules involved in amoeba adaptation to different iron conditions during the invasive process are unknown. To investigate the effects of iron availability on gene expression in E. histolytica, we determined by microarray experiments the gene expression profile of parasites grown in the presence of different iron concentrations. Conditions included low amounts of iron, iron starvation and iron starvation supplemented with hemoglobin (Hb). Genes encoding important proteins involved in iron metabolism, reported in other organism such as bacteria, were identified for the first time in E. histolytica. The influence of iron on the amount of these transcripts was confirmed by quantitative real-time PCR and their protein products were analyzed by Western blots

ORGANISM(S): Entamoeba histolytica  

SUBMITTER: Nora Hernandez  

PROVIDER: E-MTAB-1158 | ArrayExpress | 2014-06-30

REPOSITORIES: ArrayExpress

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Publications

Gene expression profiling in Entamoeba histolytica identifies key components in iron uptake and metabolism.

Hernández-Cuevas Nora Adriana NA   Weber Christian C   Hon Chung-Chau CC   Guillen Nancy N  

PloS one 20140911 9


Entamoeba histolytica is an ameboid parasite that causes colonic dysentery and liver abscesses in humans. The parasite encounters dramatic changes in iron concentration during its invasion of the host, with relatively low levels in the intestinal lumen and then relatively high levels in the blood and liver. The liver notably contains sources of iron; therefore, the parasite's ability to use these sources might be relevant to its survival in the liver and thus the pathogenesis of liver abscesses.  ...[more]

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