Transcriptomics,Multiomics

Dataset Information

93

Transcription profiling by high throughput sequencing of primordial germ cells from e6.5, e7.5, e8.5 embryos and e7.5 somatic neighbours and Blimp1-KO mice


ABSTRACT: Primordial germ cell mRNA profiles from cells microdissected from e6.5, e7.5 and e8.5 embryos, e7.5 somatic neighbours and Blimp1-KO mice were generated by single cell library construction and sequencing in duplicate using Applied Biosystems SOLiD sequencer. Single cell library construction is described in: Tang f. et. al, Nature Protocols (2010), Vol. 5, p.516.

INSTRUMENT(S): AB SOLiD System 3.0

ORGANISM(S): Mus musculus  

SUBMITTER: Sabine Dietmann   

PROVIDER: E-MTAB-1178 | ArrayExpress| 2013-07-17

SECONDARY ACCESSION(S): ERP001549

REPOSITORIES: ArrayExpress, ENA

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Publications

A tripartite transcription factor network regulates primordial germ cell specification in mice.

Magnúsdóttir Erna E   Dietmann Sabine S   Murakami Kazuhiro K   Günesdogan Ufuk U   Tang Fuchou F   Bao Siqin S   Diamanti Evangelia E   Lao Kaiqin K   Gottgens Berthold B   Azim Surani M M  

Nature cell biology 20130714 8


Transitions in cell states are controlled by combinatorial actions of transcription factors. BLIMP1, the key regulator of primordial germ cell (PGC) specification, apparently acts together with PRDM14 and AP2γ. To investigate their individual and combinatorial functions, we first sought an in vitro system for transcriptional readouts and chromatin immunoprecipitation sequencing analysis. We then integrated this data with information from single-cell transcriptome analysis of normal and mutant PG  ...[more]

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