Project description:In order to study the effect of lactate dehydrogenase (?ldh 1.2) mutant on the transcriptome DNA microarray technology was used. Stains were grown in a chemically defined medium (CDM-LAB) containing 1.1% of glucose as a carbon sources at 37?C on anaerobic condition to an optical density of 0.6.
Project description:We have studied the effects of three different growth rate (0.05h (-1), 0.15h (-1) and 0.4h(-1) on the trancriptome of E. faecalis grown under energy limitation in chemostat culture
Project description:Differences in gene content between three test strains were first assessed by comparative genomic hybridization using an E. faecalis V583 oligo array and with E. faecalis V583 as a reference strain . Transcriptional profiles of the same three test strains were then obtained through time series experiments over periods of 30min.
Project description:The effects of NaCl on transcriptional events were studied by means of genome wide microarrays in Enterococcus faecalis V583. Transcriptional profiles were obtained through time series experiments over periods of 60min.
Project description:Comparative genomic hybridization of 9 Norwegian E. faecalis baby isolates with E. faecalis V583 as a reference strain using an E. faecalis V583 oligo array. Total gene content was analyzed by whole genome microarrays.
Project description:Trancriptional response of pediocin exposure of V583 and V583 expressing immunity (petAIM), and the transcriptional response of petAIM compared to both V583 and V583 containing the vector pet.
Project description:The transcriptional responses of class IIa bacteriocin resistance in 4 mutants of Enterococcus faecalis V583; three spontaneous pediocin PA-1 mutants; MOP1, MOP2 and MOP5 and one delta-mptD mutant called MOM1, studied by microarrays in E. faecalis V583.
Project description:Gene expression of Enterococcus faecalis V583 in response to growth in 10% and 100% blood was examined by the use of of genome wide microarrays. Transcriptional profiles were obtained through time series experiments over periods of 60min (for 10% blood) and only after 30 min for growth 100% blood.