Transcriptomics

Dataset Information

238

ChIP-chip by array of budding yeast with anti-Rpb3 antibody to study how an altered RNA polymerase II (Pol II) C-terminal domain (CTD)-binding domain in Nrd1 contributes to the choice of Pol II termination pathway


ABSTRACT: The RNA polymerase II (RNApII) C-terminal domain (CTD)-interacting domain (CID) proteins are involved in two distinct termination pathways and recognize different phosphorylated forms of CTD. To investigate the role of differential CTDCID interactions in the choice of termination pathway, we altered the CTD-binding specificity of Nrd1 by domain swapping. ChIP-chip was performed to examine the effect of Nrd1 CID swapping on genome-wide RNA polymerase II (Rpb3 antibody, Neoclone) occupancy. Nrd1 with the CID from Rtt103 (Nrd1[CID-Rtt103]; strain YSB2445) causes read-through transcription at many genes, but can trigger termination where multiple Nrd1/Nab3-binding sites and serine 2 phosphorylated CTD co-exist.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Michael Lidschreiber  

PROVIDER: E-MTAB-1626 | ArrayExpress | 2013-12-04

REPOSITORIES: ArrayExpress

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Publications

The RNA polymerase II C-terminal domain-interacting domain of yeast Nrd1 contributes to the choice of termination pathway and couples to RNA processing by the nuclear exosome.

Heo Dong-hyuk DH   Yoo Inhea I   Kong Jiwon J   Lidschreiber Michael M   Mayer Andreas A   Choi Byung-Yi BY   Hahn Yoonsoo Y   Cramer Patrick P   Buratowski Stephen S   Kim Minkyu M  

The Journal of biological chemistry 20131106 51


The RNA polymerase II (RNApII) C-terminal domain (CTD)-interacting domain (CID) proteins are involved in two distinct RNApII termination pathways and recognize different phosphorylated forms of CTD. To investigate the role of differential CTD-CID interactions in the choice of termination pathway, we altered the CTD-binding specificity of Nrd1 by domain swapping. Nrd1 with the CID from Rtt103 (Nrd1(CID(Rtt103))) causes read-through transcription at many genes, but can also trigger termination whe  ...[more]

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