Transcriptomics

Dataset Information

350

RNA-seq of coding RNA of mouse long term hematopoietic stem cells, short term hematopoietic stem cells and hematopoietic multipotent progenitor cells to investigate maternal-specific imprinting in the H19-Igf2 locus


ABSTRACT: Long-term hematopoietic stem cells (HSCs), short-term HSCs and multipotent progenitor cells (MPPs) were isolated from bone marrow of four mouse strains (WT, H19-deletion, Igf1r-deletion, and double-deletion) and expression profiled with RNAseq. The behavior of the transcriptomes, and in particular the imprinted genes, was analyzed to see what might be involved in maintaining quiescence of long-term stem cells, and how H19 and Igf1r affected the expression of imprinted genes. Transcriptional profiling data of the same cells have been deposited in ArrayExpress under accession number E-MTAB-1644 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1644/).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: Ariel Paulson  

PROVIDER: E-MTAB-1628 | ArrayExpress | 2013-08-12

SECONDARY ACCESSION(S): ERP003264

REPOSITORIES: ArrayExpress, ENA

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Publications


The epigenetic regulation of imprinted genes by monoallelic DNA methylation of either maternal or paternal alleles is critical for embryonic growth and development. Imprinted genes were recently shown to be expressed in mammalian adult stem cells to support self-renewal of neural and lung stem cells; however, a role for imprinting per se in adult stem cells remains elusive. Here we show upregulation of growth-restricting imprinted genes, including in the H19-Igf2 locus, in long-term haematopoiet  ...[more]

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