Transcriptomics

Dataset Information

534

RNA-seq of non coding RNA to investigate effects of Deformed wing virus (DWV) infection and the mite Varroa destructor on siRNA and miRNA composition in the honeybee Apis mellifera


ABSTRACT: Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on on siRNA and miRNA composition using high-throughput sequencing of small RNA in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Apis mellifera  

SUBMITTER: Eugene V Ryabov  

PROVIDER: E-MTAB-1671 | ArrayExpress | 2013-09-01

SECONDARY ACCESSION(S): ERP002625

REPOSITORIES: ArrayExpress, ENA

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Publications

A virulent strain of deformed wing virus (DWV) of honeybees (Apis mellifera) prevails after Varroa destructor-mediated, or in vitro, transmission.

Ryabov Eugene V EV   Wood Graham R GR   Fannon Jessica M JM   Moore Jonathan D JD   Bull James C JC   Chandler Dave D   Mead Andrew A   Burroughs Nigel N   Evans David J DJ  

PLoS pathogens 20140626 6


The globally distributed ectoparasite Varroa destructor is a vector for viral pathogens of the Western honeybee (Apis mellifera), in particular the Iflavirus Deformed Wing Virus (DWV). In the absence of Varroa low levels DWV occur, generally causing asymptomatic infections. Conversely, Varroa-infested colonies show markedly elevated virus levels, increased overwintering colony losses, with impairment of pupal development and symptomatic workers. To determine whether changes in the virus populati  ...[more]

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