Transcriptomics

Dataset Information

533

RNA-seq of Deformed wing virus (DWV) cDNA fragments covering complete DWV genomic RNA sequences extracted from virus preparations and the injected honeybee (Apis mellifera) pupae


ABSTRACT: Experiment was designed (i) to analyse the strain composition of Deformed wing virus (DWV) populations in covertly and overtly infected honeybees (Apis mellifera) from Varroa-free and Varroa-infested colonies, and (ii) to determine abundance of the DWV strains following direct injection of the DWV preparations from covertly and overtly infected bees to the bee pupae haemolymph in the absence of Varroa destructor mites. Experiment included isolation of DWV preparations from the following bees: covertly-infected bees from Varroa-free colony, covertly infected bees exposed orally to the Varroa-selected DWV strains, and the overtly infected Varroa-exposed bees. Honeybee pupae were experimentally injected with those DWV preparations and sampled 4 days post injection following development of overt DWV infection. A series of the DWV cDNA fragment covering complete DWV genomic RNA sequences were amplified by RT-PCR using RNA extracted from virus preparations and the injected pupae. The cDNA preparations were sequenced using next generation(Illumina HighSeq 2000) paired-end sequencing to obtain data on the DWV strain composition.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Deformed wing virus  

SUBMITTER: Eugene V Ryabov  

PROVIDER: E-MTAB-1675 | ArrayExpress | 2013-09-01

SECONDARY ACCESSION(S): ERP002660

REPOSITORIES: ArrayExpress, ENA

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Publications

A virulent strain of deformed wing virus (DWV) of honeybees (Apis mellifera) prevails after Varroa destructor-mediated, or in vitro, transmission.

Ryabov Eugene V EV   Wood Graham R GR   Fannon Jessica M JM   Moore Jonathan D JD   Bull James C JC   Chandler Dave D   Mead Andrew A   Burroughs Nigel N   Evans David J DJ  

PLoS pathogens 20140626 6


The globally distributed ectoparasite Varroa destructor is a vector for viral pathogens of the Western honeybee (Apis mellifera), in particular the Iflavirus Deformed Wing Virus (DWV). In the absence of Varroa low levels DWV occur, generally causing asymptomatic infections. Conversely, Varroa-infested colonies show markedly elevated virus levels, increased overwintering colony losses, with impairment of pupal development and symptomatic workers. To determine whether changes in the virus populati  ...[more]

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