Transcriptomics

Dataset Information

244

Transcription profiling by array of individual inner cells during mouse blastocyst development


ABSTRACT: Mammalian embryonic development begins with the specification and segregation of the two extra-embryonic lineages, trophectoderm and primitive endoderm, from the pluripotent embryonic lineage, the epiblast. To establish a map of epiblast (EPI) versus primitive endoderm (PrE) lineage segregation which occurs within the inner cell mass (ICM), we comprehensively characterised the gene expression profiles of individual inner cells during blastocyst development. Lineage represents the two embryonic cell lineages: the epiblast (EPI), and the primitive endoderm (PE), which are segregated within the inner cell mass(ICM) during blastocyst development.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Wolfgang Huber   Andrzej K. Oleś  

PROVIDER: E-MTAB-1681 | ArrayExpress | 2013-11-26

REPOSITORIES: ArrayExpress

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Publications

Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages.

Ohnishi Yusuke Y   Huber Wolfgang W   Tsumura Akiko A   Kang Minjung M   Xenopoulos Panagiotis P   Kurimoto Kazuki K   Oleś Andrzej K AK   Araúzo-Bravo Marcos J MJ   Saitou Mitinori M   Hadjantonakis Anna-Katerina AK   Hiiragi Takashi T  

Nature cell biology 20131201 1


It is now recognized that extensive expression heterogeneities among cells precede the emergence of lineages in the early mammalian embryo. To establish a map of pluripotent epiblast (EPI) versus primitive endoderm (PrE) lineage segregation within the inner cell mass (ICM) of the mouse blastocyst, we characterized the gene expression profiles of individual ICM cells. Clustering analysis of the transcriptomes of 66 cells demonstrated that initially they are non-distinguishable. Early in the segre  ...[more]

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