Genomics

Dataset Information

299

ChIP-seq of five human cell lines and mouse liver samples with various antibodies to validate the ChIP-exo protocol (ligation of TruSeq P7 exo-adapter to end-repaired immunoprecipitated chromatin)


ABSTRACT: This experiment validates the ChIP-exo protocol on the Illumina sequencing platform.

INSTRUMENT(S): Illumina Genome Analyzer IIx, Illumina HiSeq 2000

ORGANISM(S): Musculus  

SUBMITTER: Gordon D Brown  

PROVIDER: E-MTAB-1827 | ArrayExpress | 2014-01-15

SECONDARY ACCESSION(S): ERP003828

REPOSITORIES: ArrayExpress, ENA

altmetric image

Publications

Development of an Illumina-based ChIP-exonuclease method provides insight into FoxA1-DNA binding properties.

Serandour Aurelien A AA   Brown Gordon D GD   Cohen Joshua D JD   Carroll Jason S JS  

Genome biology 20131227 12


ChIP-exonuclease (ChIP-exo) is a modified ChIP-seq approach for high resolution mapping of transcription factor DNA sites. We describe an Illumina-based ChIP-exo method which provides a global improvement of the data quality of estrogen receptor (ER) ChIP and insights into the motif structure for key ER-associated factors. ChIP-exo of the ER pioneer factor FoxA1 identifies protected DNA with a predictable 8 bp overhang from the Forkhead motif, which we term mesas. We show that mesas occur in mul  ...[more]

Publication: 1/2

Similar Datasets

2013-07-17 | E-GEOD-48930 | ArrayExpress
2014-04-17 | E-MTAB-1694 | ArrayExpress
2014-01-14 | E-MTAB-1749 | ArrayExpress
2014-05-10 | E-MTAB-2060 | ArrayExpress
2012-04-01 | E-MTAB-959 | ArrayExpress
2018-03-06 | E-MTAB-5769 | ArrayExpress
2013-03-21 | E-MTAB-1104 | ArrayExpress
2013-01-18 | E-GEOD-41561 | ArrayExpress
2014-09-27 | E-MTAB-1509 | ArrayExpress
2015-03-02 | E-GEOD-58013 | ArrayExpress