Dataset Information


ChIP-seq of five human cell lines and mouse liver samples with various antibodies to validate the ChIP-exo protocol (ligation of TruSeq P7 exo-adapter to end-repaired immunoprecipitated chromatin)

ABSTRACT: This experiment validates the ChIP-exo protocol on the Illumina sequencing platform.

INSTRUMENT(S): Illumina Genome Analyzer IIx, Illumina HiSeq 2000

ORGANISM(S): Musculus  

SUBMITTER: Gordon D Brown  

PROVIDER: E-MTAB-1827 | ArrayExpress | 2014-01-15



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Development of an Illumina-based ChIP-exonuclease method provides insight into FoxA1-DNA binding properties.

Serandour Aurelien A AA   Brown Gordon D GD   Cohen Joshua D JD   Carroll Jason S JS  

Genome biology 20131227 12

ChIP-exonuclease (ChIP-exo) is a modified ChIP-seq approach for high resolution mapping of transcription factor DNA sites. We describe an Illumina-based ChIP-exo method which provides a global improvement of the data quality of estrogen receptor (ER) ChIP and insights into the motif structure for key ER-associated factors. ChIP-exo of the ER pioneer factor FoxA1 identifies protected DNA with a predictable 8 bp overhang from the Forkhead motif, which we term mesas. We show that mesas occur in mul  ...[more]

Publication: 1/2

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